Luteolin as a potent anti-leishmanial agent against intracellular Leishmania tropica parasite

Purpose: To examine the anti-leishmanial and cytotoxic effects of five naturally occurring phenolic compounds: luteolin (1), lalioside (2), luteolin-4’-O-β-D-glucopyranoside (3), apigenin 4-O-β-Dglucopyranoside (4) and apigenin (5) on Leishmania tropica KWH23 amastigotes .Methods: The compounds were isolated from the leaves of Lawsonia Inermis via hyphenated high performance liquid chromatography-high resolution mass spectrometry coupled with solid phase extraction-tube transfer nuclear magnetic resonance technique. The isolated compounds were given intraperitoneally to L. tropica KWH23 amastigotes-infected albino mice at a dose of ≥ 3 mg/kg for 5 days. Amphotericin-B was used as standard (reference) drug. Lymphocytes were used to analyze their cytotoxicity.Results: For compound 1, mean lesion size decreased from 0.82 ± 0.12 to 0.10 ± 0.01 after 120 days, with 97 % cure of intracellular L. tropica amastigotes at a dose of 15 mg/kg, compared to amphotericin B which produced 95 % cure at a dose of 30 mg/kg. Half-maximal concentration (IC50) for compound 1 was 4.15 μg/ml against lymphocytes.Conclusion: The results indicate that luteolin is a potent inhibitor of L. tropica  amastigotes, with a higher cytotoxic activity against lymphocytes, compared with luteolin-4’-O-β-D-glucopyranoside.Keywords: Leishmania tropica, Luteolin, Lalioside, Luteolin-4’-O-β-D-glucopyranoside, Apigenin 4-O- β-D-glucopyranoside, Apigeni

On the Relationship Between the Fractional Sumudu Transform and Fractional Fourier Transform

In this paper we have discussed fractional Sumudu transforms and its relationship with fractional Fourier transform and obtained the mathematical expression of kernel of fractional Sumudu transform. Such findings  will play a significant role for fractional Sumudu transform to recognize its importance in the  fields of engineering and applied mathematics  like other fractional transforms

Positive Organizational Study: A Comparison between Private and Government University Teacher

The study conducted explored the relation between Psychological Capital, General Health and Organizational Citizenship Behavior and its variation between the teachers of private and government universities of Karachi, Pakistan. Based on existing literature, following hypotheses were formulated; (1) Difference would be observed in psychological capital of private and government university teachers, (2) Difference would be observed in efficacy levels of private and government university teachers, (3) Individuals with good health will have high psychological capital, (4) Individuals with high OCB will have high psychological capital. Through convenient sampling, 261 teachers teaching at graduate-level were taken as participants. Psychological Capital Questionnaire, General Health Questionnaire and Organizational Citizenship Behavior Checklist were employed. All the hypotheses were proved when results were analyzed through SPSS. In conclusion, people having high psychological capital live healthier life and are more likely to indulge in pro-social activities and organizational citizenship behavior

Surgical site infection; effect of contamination and duration of surgical procedure

Introduction: Surgical site infections are responsible for increased treatment cost, prolonged hospital stay and increased morbidity on surgical floor. Increased level of per operative contamination and prolonged surgery increases the incidence of surgical site infections.Objective: To know the effect of contamination and duration of surgery on the incidence of surgical site infections in emergency surgical patients so that specific strategies can be developed to decrease the morbidity and mortality caused by these infections.Materials and Methods: All the patients who underwent general surgical operations on an emergency basis at the surgical unit-1 of the Benazir Bhutto Hospital Rawalpindi from 01-01-2019 to 31-12-2020 were evaluated for surgical site infections. The level of contamination per operatively and duration of surgery were documented. Surgical site infections suspected clinically were confirmed by culture and sensitivity.Results: Among 2202 emergency-operated patients, two hundred and thirty-seven patients (10.76%) had surgical site infection confirmed on culture and sensitivity (C/S) report. About sixty-five percent of patients were male. Of two hundred and thirty-seven positive patients, seventy (29.09%) patients underwent laparotomy for penetrating and blunt abdominal trauma. About fifty-eight percent of SSI patients had contaminated wounds per operatively. Operative time was one to three hours in about sixty-seven percent of SSI-positive patients. Staph aureus was present in one hundred and forty-five (79.67%) patients. E.coli was the most commonest Gram-ve micro-organism (70.95%).Conclusion: Surgical site infection causes a significant rise in morbidity on the surgical floor. Increased levels of contamination per operatively and prolonged operative time increase the incidence of SSI in emergency surgical operations

Surgical site infection; effect of contamination and duration of surgical procedure

Introduction: Surgical site infections are responsible for increased treatment cost, prolonged hospital stay and increased morbidity on surgical floor. Increased level of per operative contamination and prolonged surgery increases the incidence of surgical site infections.Objective: To know the effect of contamination and duration of surgery on the incidence of surgical site infections in emergency surgical patients so that specific strategies can be developed to decrease the morbidity and mortality caused by these infections.Materials and Methods: All the patients who underwent general surgical operations on an emergency basis at the surgical unit-1 of the Benazir Bhutto Hospital Rawalpindi from 01-01-2019 to 31-12-2020 were evaluated for surgical site infections. The level of contamination per operatively and duration of surgery were documented. Surgical site infections suspected clinically were confirmed by culture and sensitivity.Results: Among 2202 emergency-operated patients, two hundred and thirty-seven patients (10.76%) had surgical site infection confirmed on culture and sensitivity (C/S) report. About sixty-five percent of patients were male. Of two hundred and thirty-seven positive patients, seventy (29.09%) patients underwent laparotomy for penetrating and blunt abdominal trauma. About fifty-eight percent of SSI patients had contaminated wounds per operatively. Operative time was one to three hours in about sixty-seven percent of SSI-positive patients. Staph aureus was present in one hundred and forty-five (79.67%) patients. E.coli was the most commonest Gram-ve micro-organism (70.95%).Conclusion: Surgical site infection causes a significant rise in morbidity on the surgical floor. Increased levels of contamination per operatively and prolonged operative time increase the incidence of SSI in emergency surgical operations

Power of Linguistic Privilege: Critical Discourse Analysis of the Narratives of Pakistani Immigrant Students in American Schools

Abstract This paper highlights the struggle that Pakistani immigrant students make during the process of learning English as a second language and the ways in which lack of proficiency in English complicates the process of learning and even hinders their academic growth. The major focus is on immigrant students at the beginning level of English language learning who come from less commonly known cultures and with their first language less commonly taught in USA. Discourse analysis of the narratives of three Pakistani immigrant students is presented and discussed in the context of language, identity and power relation